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5 October 2023 Cilia function as calcium-mediated mechanosensors that instruct left-right asymmetry
Mohammed Mahamdeh, Lydia Djenoune, Thai V. Truong, Christopher T. Nguyen, Scott E. Fraser, Martina Brueckner, Jonathon Howard, Shiaulou Yuan
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Proceedings Volume PC12649, Optical Trapping and Optical Micromanipulation XX; PC126491K (2023) https://doi.org/10.1117/12.2680858
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States
Abstract
The breaking of bilateral symmetry in most vertebrates is critically dependent upon the motile cilia of the embryonic left-right organizer (LRO), which generate a directional fluid flow; however, it remains unclear how this flow is sensed. Here, we demonstrated that immotile LRO cilia are mechanosensors for shear force using a methodological pipeline that combines optical tweezers, light sheet microscopy, and deep learning to permit in vivo analyses in zebrafish. Mechanical manipulation of immotile LRO cilia activated intraciliary calcium transients that required the cation channel Polycystin-2. Furthermore, mechanical force applied to LRO cilia was sufficient to rescue and reverse cardiac situs in zebrafish that lack motile cilia. Thus, LRO cilia are mechanosensitive cellular levers that convert biomechanical forces into calcium signals to instruct left-right asymmetry.
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Mohammed Mahamdeh, Lydia Djenoune, Thai V. Truong, Christopher T. Nguyen, Scott E. Fraser, Martina Brueckner, Jonathon Howard, and Shiaulou Yuan "Cilia function as calcium-mediated mechanosensors that instruct left-right asymmetry", Proc. SPIE PC12649, Optical Trapping and Optical Micromanipulation XX, PC126491K (5 October 2023); https://doi.org/10.1117/12.2680858
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KEYWORDS
Calcium
Biomechanics
Cardiovascular disorders
Heart
Machine learning
Microscopy
Optical tweezers
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