Limits of detection for the analysis of in vivo skin penetration of topical products by Raman spectroscopy

Tom C. Bakker-Schut; Gerwin J. Puppels; Claudio Nico; Peter J Caspers

doi:10.1117/12.3002399

13 March 2024 Limits of detection for the analysis of in vivo skin penetration of topical products by Raman spectroscopy

Tom C. Bakker-Schut, Gerwin J. Puppels, Claudio Nico, Peter J Caspers

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Proceedings Volume PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII; PC128210E (2024) https://doi.org/10.1117/12.3002399
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The limit of detection (LoD) is the lowest concentration of an analyte that can be reliably observed with a sufficient degree of confidence. This presentation will discuss a method to determine the LoD for detecting an exogenous analyte in the Stratum Corneum, using classical Raman spectroscopy and a least-squares fit analysis method. We will show how measurement dependent parameters like the signal-to-noise of the spectra, available spectral information, and the quality of the fit influence the LoD.

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Tom C. Bakker-Schut, Gerwin J. Puppels, Claudio Nico, and Peter J Caspers "Limits of detection for the analysis of in vivo skin penetration of topical products by Raman spectroscopy", Proc. SPIE PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, PC128210E (13 March 2024); https://doi.org/10.1117/12.3002399

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KEYWORDS

Skin

Raman spectroscopy

In vivo imaging

Raman scattering

Signal to noise ratio

Spectral models

Toxicology

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