CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC12831 > Article
Presentation
13 March 2024 A miniaturized microscope for real-time monitoring of leukocytes and noninvasive diagnosis of inflammation
Arutyun Bagramyan, Juwell Wu, Charles P. Lin
Author Affiliations +
Proceedings Volume PC12831, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII; PC128310M (2024) https://doi.org/10.1117/12.3001636
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Complete blood cell count (CBC) is one of the most valuable clinical tests to measure alternations in leukocyte numbers and to provide signs of infection and severe inflammation (e.g., sepsis). However, the CBC procedure is invasive, often requiring multiple blood samples for ex vivo laboratory analysis. Moreover, it does not provide dynamic cell information such as enhanced adhesiveness of leukocytes on the blood vessel walls, a cardinal feature of inflammation. We have developed a miniaturized oblique back-illumination microscope capable of noninvasive, high-resolution and label-free imaging of human microvasculature. The instrument was used to image and quantify circulating leukocytes, and the results are compared with CBC. We also investigated the leukocyte-endothelial interactions in inflamed conditions, revealing a drastic change in cell rolling speed. To provide clinicians with reliable and actionable results, we are developing a deep learning algorithm for automated quantification and movement analysis.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Arutyun Bagramyan, Juwell Wu, and Charles P. Lin "A miniaturized microscope for real-time monitoring of leukocytes and noninvasive diagnosis of inflammation", Proc. SPIE PC12831, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII, PC128310M (13 March 2024); https://doi.org/10.1117/12.3001636
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
White blood cells
Miniaturization
Microscopes
Inflammation
Blood
Equipment
Algorithm development
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top