Elucidating biophysical mechanisms using quantitative phase imaging to track transmembrane water flux in living cells

Sean P. O'Connor; Anna V. Sedelnikova; Matthew T. Conway; Stacey L. Martens; Allen S. Kiester; Joel N. Bixler; Zachary A. Steelman

doi:10.1117/12.2688768

13 March 2024 Elucidating biophysical mechanisms using quantitative phase imaging to track transmembrane water flux in living cells

Sean P. O'Connor, Anna V. Sedelnikova, Matthew T. Conway, Stacey L. Martens, Allen S. Kiester, Joel N. Bixler, Zachary A. Steelman

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Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128520O (2024) https://doi.org/10.1117/12.2688768
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We apply our recently developed QPI methodologies, which quantify and track intracellular water content, to investigate the role of aquaporin (AQP) proteins in pulsed electric field (PEF) induced water uptake in cells. QPI imaging is performed on Jurkat and glial cells subjected to PEF simulation. The effects on the transmembrane water flux of the cells are investigated when solutions contain mercury, a known broad spectrum AQP blocker, or commercially available AQP blockers. Our study demonstrates the utility of QPI for rapid intracellular water quantification and shows a path forward for identifying biophysical mechanisms using label-free imaging.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Sean P. O'Connor, Anna V. Sedelnikova, Matthew T. Conway, Stacey L. Martens, Allen S. Kiester, Joel N. Bixler, and Zachary A. Steelman "Elucidating biophysical mechanisms using quantitative phase imaging to track transmembrane water flux in living cells", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128520O (13 March 2024); https://doi.org/10.1117/12.2688768

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KEYWORDS

Phase imaging

Electric fields

Glia

Mercury

Phase shifts

Proteins

Spherical lenses

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