Unveiling the dynamics and structure of eukaryotic flagella: fast and precise 3D tracking using digital holographic microscopy

Patryk Nienaltowski; Jonasz Slomka; Clara Martinez-Perez; Tristan Colomb; Yves Emery; Roman Stocker

doi:10.1117/12.3001870

13 March 2024 Unveiling the dynamics and structure of eukaryotic flagella: fast and precise 3D tracking using digital holographic microscopy

Patryk Nienaltowski, Jonasz Slomka, Clara Martinez-Perez, Tristan Colomb, Yves Emery, Roman Stocker

Author Affiliations +

Proceedings Volume PC12852, Quantitative Phase Imaging X; PC128521D (2024) https://doi.org/10.1117/12.3001870
Event: SPIE BiOS, 2024, San Francisco, California, United States

Conference Poster

Abstract

The flagellum is vital for eukaryotic cell survival. We propose method utilizing digital holographic microscopy (DHM) advantages, such as high-speed imaging and precise axial localization, to develop a versatile approach for four-dimensional tracking (X, Y, Z, time) of eukaryotic microorganisms' bodies and flagella, addressing challenges faced by existing methods. We reconstructed for the first time the shape of a 200 nm diameter Chrysochromulina simplex flagellum and measured mouse sperm flagella over time, capturing approximately 800 points. Our technique opens new avenues for studying flagella's roles in cellular functions and survival strategies, offering high-speed and precise 3D tracking at the nanoscale.

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Patryk Nienaltowski, Jonasz Slomka, Clara Martinez-Perez, Tristan Colomb, Yves Emery, and Roman Stocker "Unveiling the dynamics and structure of eukaryotic flagella: fast and precise 3D tracking using digital holographic microscopy", Proc. SPIE PC12852, Quantitative Phase Imaging X, PC128521D (13 March 2024); https://doi.org/10.1117/12.3001870

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