Label-free cellular imaging of fresh ophthalmic tissues with dual-mode full-field optical coherence tomography

Peng Xiao; Keyi Fei; Bingying Lin; Jinze Zhang; Yushuang Liu; Zhongzhou Luo; Jin Yuan

doi:10.1117/12.2692428

13 March 2024 Label-free cellular imaging of fresh ophthalmic tissues with dual-mode full-field optical coherence tomography

Peng Xiao, Keyi Fei, Bingying Lin, Jinze Zhang, Yushuang Liu, Zhongzhou Luo, Jin Yuan

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540B (2024) https://doi.org/10.1117/12.2692428
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The structural and functional imaging of ophthalmic tissues in cellular level play an important role in the understanding and evaluation of the physiology and pathology of ophthalmic diseases. In this study, we developed a dual-mode full-field optical coherence tomography (FFOCT) that is capable of acquiring label-free cellular images of freshly excised ophthalmic tissues, achieving static contrasts gained from structural refractive index gradients and dynamic contrast induced by endogenous cell motility related to cell functions. Through imaging experiments on both normal and pathological ophthalmic tissues, we show that while the static FFOCT images better reveal the relative stationary cellular structures like nerve fibers and collagens, the dynamic FFOCT images show enhanced contrast of various transparent cells with active intracellular metabolic motions, offering complementary information of major corneal and retinal layers. Our study has shown the dual-mode FFOCT system is a straightforward promising technique for cellular imaging exploration and pathological analysis of ophthalmic tissues.

Conference Presentation

Citation Download Citation

Peng Xiao, Keyi Fei, Bingying Lin, Jinze Zhang, Yushuang Liu, Zhongzhou Luo, and Jin Yuan "Label-free cellular imaging of fresh ophthalmic tissues with dual-mode full-field optical coherence tomography", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540B (13 March 2024); https://doi.org/10.1117/12.2692428

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KEYWORDS

Tissues

Optical coherence tomography

Imaging systems

Interferometry

Pathology

Retina

Signal analyzers

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