Single capture quantitative oblique back-illumination microscopy enables in-vivo quantitative phase imaging

Paloma Casteleiro Costa; Jamin Ahn; Anthony Lien; Mercedes López-Esteva; Bilal Haider; Francisco E. Robles

doi:10.1117/12.3001865

13 March 2024 Single capture quantitative oblique back-illumination microscopy enables in-vivo quantitative phase imaging

Paloma Casteleiro Costa, Jamin Ahn, Anthony Lien, Mercedes López-Esteva, Bilal Haider, Francisco E. Robles

Author Affiliations +

Proceedings Volume PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024; PC128540U (2024) https://doi.org/10.1117/12.3001865
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Quantitative Oblique Back-Illumination Microscopy (qOBM) enables 3D quantitative phase imaging (QPI) in thick scattering samples. Recently, we developed a deep learning assisted single-capture qOBM (SC-qOBM) method to achieve fast quantitative phase tomography in vivo. This novel approach paves the way for a number of in-vivo applications that were previously out of reach for QPI. Here, we showcase two applications of SC-qOBM: (1) in-vivo imaging of blood flow in mouse brains for studying blood diseases and (2) non-invasive imaging of blood flow in humans for hematological and in-vivo flow cytometry analysis. This work highlights SC-qOBM’s potential in various biomedical applications.

Conference Presentation

Citation Download Citation

Paloma Casteleiro Costa, Jamin Ahn, Anthony Lien, Mercedes López-Esteva, Bilal Haider, and Francisco E. Robles "Single capture quantitative oblique back-illumination microscopy enables in-vivo quantitative phase imaging", Proc. SPIE PC12854, Label-free Biomedical Imaging and Sensing (LBIS) 2024, PC128540U (13 March 2024); https://doi.org/10.1117/12.3001865

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