Fluorescence quenching effects on decoherence and energy transfer dynamics of green fluorescent proteins

Anna Cusick; Jacqueline Li; Alejandro Jimenez; Youngchan Kim

doi:10.1117/12.3002389

13 March 2024 Fluorescence quenching effects on decoherence and energy transfer dynamics of green fluorescent proteins

Anna Cusick, Jacqueline Li, Alejandro Jimenez, Youngchan Kim

Proceedings Volume PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics; PC128630V (2024) https://doi.org/10.1117/12.3002389
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

The study of decoherence in biological systems has become an essential area of research for quantum mechanics. Fluorescence quenching is a physiochemical process that causes the decrease in fluorescence intensity of a fluorescent sample. Changing the surrounding environment of fluorescent proteins (FPs), by adding a fluorescence quenching agent could potentially have a large impact on the dynamical processes within these biological systems. Environmental influence on protein-protein interactions is not a well understood process, when looking at energy transfer dynamics of FPs. we attempt to establish the impact on the energy transfer dynamics between fluorophores by introducing varying concentrations of external quenchers to the FPs environment. We examine the photophysical properties and spectral changes of the FPs, after adding external quenchers to both the monomeric and dimeric structures to further characterise the energy transfer dynamics.

Conference Presentation

Citation Download Citation

Anna Cusick, Jacqueline Li, Alejandro Jimenez, and Youngchan Kim "Fluorescence quenching effects on decoherence and energy transfer dynamics of green fluorescent proteins", Proc. SPIE PC12863, Quantum Effects and Measurement Techniques in Biology and Biophotonics, PC128630V (13 March 2024); https://doi.org/10.1117/12.3002389

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