CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC13006 > Article
Presentation
20 June 2024 Sensing forces in cells using fluorescence FRET microscopy and optical tweezers
Camille Dubois, Nathalie Westbrook, Nada Boustany, Marie Erard, Ludivine Houel-Renault
Author Affiliations +
Proceedings Volume PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III; PC130060Q (2024) https://doi.org/10.1117/12.3029460
Event: SPIE Photonics Europe, 2024, Strasbourg, France
Abstract
Forces inside cells play a fundamental role in cell behavior, for example in cancer cell migration. We focus on the vinculin protein which is involved in the stabilization of cell adhesion. Through fluorescence transfer (FRET), forces within vinculin can be measured with picoNewton sensitivity. We measure these internal forces while applying a calibrated external force with a laser-based optical tweezer via a microbead attached to the cell. Our most recent results using fibroblast cells show that the force applied with the optical tweezer induces the recruitment of vinculin and the formation of focal adhesions on the bead within a few minutes. Once the bead is attached to the cell, we record its trajectory and infer the force exerted by the cell. We correlate this force with the FRET efficiency of the force sensor.
Conference Presentation
© (2024) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Camille Dubois, Nathalie Westbrook, Nada Boustany, Marie Erard, and Ludivine Houel-Renault "Sensing forces in cells using fluorescence FRET microscopy and optical tweezers", Proc. SPIE PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III, PC130060Q (20 June 2024); https://doi.org/10.1117/12.3029460
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Fluorescence resonance energy transfer
Optical tweezers
Fluorescence
Focal adhesions
Optical microscopy
Proteins
Sensors
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top