Extending two photon scanning microscopy for imaging on novel species: brain-wide in vivo imaging in Xenopus tadpoles

Filip Janiak; Carola Yovanovich; Michael Forsthofer; David Vijatovic; Lora Sweeney; Tom Baden

doi:10.1117/12.3029585

20 June 2024 Extending two photon scanning microscopy for imaging on novel species: brain-wide in vivo imaging in Xenopus tadpoles

Filip Janiak, Carola Yovanovich, Michael Forsthofer, David Vijatovic, Lora Sweeney, Tom Baden

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Proceedings Volume PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III; PC130060R (2024) https://doi.org/10.1117/12.3029585
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

In neuroscience, two photon scanning microscopy is commonly used to record brain activity in species that differ greatly in brain size and their properties and distributions of neurons. Accordingly, tailoring the properties of the imaging system to the experimental model in question is critical. These include adjustments in the size of the field-of-view, the shape and curvature of the scan-plane, or adjustment in excitation PSF sizes. Here, we report our progress to optimising these and other imaging parameters for high-signal-to-noise imaging of whole-brain neuronal activity in the large nervous system of Xenopus laevis tadpoles with comparable quality compared to what is currently possible in juvenile zebrafish.

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Filip Janiak, Carola Yovanovich, Michael Forsthofer, David Vijatovic, Lora Sweeney, and Tom Baden "Extending two photon scanning microscopy for imaging on novel species: brain-wide in vivo imaging in Xenopus tadpoles", Proc. SPIE PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III, PC130060R (20 June 2024); https://doi.org/10.1117/12.3029585

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KEYWORDS

In vivo imaging

Photonic microstructures

Brain

Imaging systems

Neuroimaging

Point spread functions

Sensors

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