Longitudinal multimodal imaging for tracking stem cells in the retina

Van Phuc Nguyen; Xueding Wang; Yannis M. Paulus

doi:10.1117/12.3017101

20 June 2024 Longitudinal multimodal imaging for tracking stem cells in the retina

Van Phuc Nguyen, Xueding Wang, Yannis M. Paulus

Proceedings Volume PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III; PC1300613 (2024) https://doi.org/10.1117/12.3017101
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Stem cell therapy holds tremendous potential for treating a wide range of currently incurable diseases, including retinal degenerations and geographic atrophy from macular degeneration. This study aimed to develop a non-invasive multimodality imaging system to track stem cells after transplantation in the subretinal space. Human-induced pluripotent stem cells differentiated to retinal pigment epithelium (hiPSC-RPE) cells were labeled with ultraminiaturized gold nanochains. The labeled hiPSC-RPE cells were then injected into 21 rabbits at 4 days after laser-induced photocoagulation damage to the RPE. Color fundus photography, photoacoustic microscopy, optical coherence tomography and fluorescent imaging were used to monitor the rabbit retina before and after the transplantation. The migration pattern and viability of the cells were monitored up to 6 months with a 37-fold increase in 650nm PAM signal. This approach allowed for the longitudinal evaluation of location of the transplanted stem cells, providing valuable insights for the advancement of stem cell therapies.

Conference Presentation

Citation Download Citation

Van Phuc Nguyen, Xueding Wang, and Yannis M. Paulus "Longitudinal multimodal imaging for tracking stem cells in the retina", Proc. SPIE PC13006, Biomedical Spectroscopy, Microscopy, and Imaging III, PC1300613 (20 June 2024); https://doi.org/10.1117/12.3017101

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