Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence

Alain Bouchard; Julie Frechette; Marcia L. Vernon; Jean-François Cormier; Rene M. Beaulieu; Réal Vallée; Akier Assanta Mafu

doi:10.1117/1.2162166

1 January 2006 Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence

Alain Bouchard, Julie Frechette, Marcia L. Vernon, Jean-François Cormier, Rene M. Beaulieu, Réal Vallée, Akier Assanta Mafu

Author Affiliations +

Journal of Biomedical Optics, Vol. 11, Issue 1, 014011 (January 2006). https://doi.org/10.1117/1.2162166

Abstract

A scanning optical system for the detection of bacteria on meat surfaces based on fluorescence lifetime and intensity measurements is described. The system detects autofluorescent light emitted by naturally occurring fluorophores in bacteria. The technique only requires minimal sample preparation and handling, thus the chemical properties of the specimen are preserved. This work presents the preliminary results obtained from a time-resolved fluorescence imaging system for the characterization of a nonpathogenic gram-negative bacteria, Pseudomonas fluorescens. Initial results indicate that the combination of fluorescence lifetime and intensity measurements provides a means for characterizing biological media and for detecting microorganisms on surfaces.

©(2006) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Alain Bouchard, Julie Frechette, Marcia L. Vernon, Jean-François Cormier, Rene M. Beaulieu, Réal Vallée, and Akier Assanta Mafu "Optical characterization of Pseudomonas fluorescens on meat surfaces using time-resolved fluorescence," Journal of Biomedical Optics 11(1), 014011 (1 January 2006). https://doi.org/10.1117/1.2162166

Published: 1 January 2006

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 5 scholarly publications and 15 patents.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Data modeling

Bacteria

Biomedical optics

Imaging systems

Time resolved spectroscopy

Microorganisms

Show All Keywords

RELATED CONTENT

Superiorities of time correlated single photon counting against standard fluorimetry...
Proceedings of SPIE (May 06 2015)

Bioluminescence tomography improves quantitative accuracy for pre-clinical imaging
Proceedings of SPIE (June 14 2013)

Food quality inspection by speckle decorrelation properties of bacteria colonies
Proceedings of SPIE (June 26 2017)

Laguerre nonparametric deconvolution technique of time resolved fluorescence data ...
Proceedings of SPIE (July 13 2004)

Life on Mars: new evidence from martian meteorites
Proceedings of SPIE (September 10 2009)

Non-contact characterization of bacteria by time-resolved fluorescence
Proceedings of SPIE (July 01 2004)

Applications of immunomagnetic capture and time resolved fluorescence detection for...
Proceedings of SPIE (April 15 2008)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years