Hybrid phosphorescence and fluorescence native spectroscopy for breast cancer detection

Alexandra N. Alimova; Alvin Katz; Vidyasagar Sriramoju; Yuri Budansky; Alexei A. Bykov; Roman Zelikovitch; Robert R. Alfano

doi:10.1117/1.2437139

1 January 2007 Hybrid phosphorescence and fluorescence native spectroscopy for breast cancer detection

Alexandra N. Alimova, Alvin Katz, Vidyasagar Sriramoju, Yuri Budansky, Alexei A. Bykov, Roman Zelikovitch, Robert R. Alfano

Author Affiliations +

Journal of Biomedical Optics, Vol. 12, Issue 1, 014004 (January 2007). https://doi.org/10.1117/1.2437139

Abstract

Fluorescence and phosphorescence measurements are performed on normal and malignant ex vivo human breast tissues using UV LED and xenon lamp excitation. Tryptophan (trp) phosphorescence intensity is higher in both normal glandular and adipose tissue when compared to malignant tissue. An algorithm based on the ratio of trp fluorescence intensity at 345 nm to phosphorescence intensity at 500 nm is successfully used to separate normal from malignant tissue types. Normal specimens consistently exhibited a low I₃₄₅/I₅₀₀ ratio (<10), while for malignant specimens, the I₃₄₅/I₅₀₀ ratio is consistently high (>15). The ratio analysis correlates well with histopathology. Intensity ratio maps with a spatial resolution of 0.5 mm are generated in which local regions of malignancy could be identified.

©(2007) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Alexandra N. Alimova, Alvin Katz, Vidyasagar Sriramoju, Yuri Budansky, Alexei A. Bykov, Roman Zelikovitch, and Robert R. Alfano "Hybrid phosphorescence and fluorescence native spectroscopy for breast cancer detection," Journal of Biomedical Optics 12(1), 014004 (1 January 2007). https://doi.org/10.1117/1.2437139

Published: 1 January 2007

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 23 scholarly publications.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tissues

Phosphorescence

Luminescence

Breast

Fluorescence spectroscopy

Proteins

Lamps

Show All Keywords

RELATED CONTENT

Study of the fluorescence signal for gastrointestinal dysplasia detection
Proceedings of SPIE (August 22 2014)

Fluorescence spectroscopy using indocyanine green for lymph node mapping
Proceedings of SPIE (February 27 2014)

Label free pathological evaluation of grade 3 cancer using Stokes...
Proceedings of SPIE (March 11 2016)

Correlation of metabolites in saliva and in vivo tissue of...
Proceedings of SPIE (February 21 2020)

UV fluorescence spectroscopic technique in the diagnosis of breast, ovarian,...
Proceedings of SPIE (June 01 1991)

Hybrid native phosphorescence and fluorescence spectroscopy for cancer detection
Proceedings of SPIE (February 22 2006)

Compact Stokes shift and fluorescence spectroscopic diagnostics LED ratiometer unit...
Proceedings of SPIE (February 16 2012)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years