Nonnegative matrix factorization: a blind spectra separation method for in vivo fluorescent optical imaging

Anne-Sophie Montcuquet; Lionel Hervé; Fabrice P. Navarro Y Garcia; Jean-Marc Dinten; Jérôme I. Mars

doi:10.1117/1.3491796

1 September 2010 Nonnegative matrix factorization: a blind spectra separation method for in vivo fluorescent optical imaging

Anne-Sophie Montcuquet, Lionel Hervé, Fabrice P. Navarro Y Garcia, Jean-Marc Dinten, Jérôme I. Mars

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Journal of Biomedical Optics, Vol. 15, Issue 5, 056009 (September 2010). https://doi.org/10.1117/1.3491796

Abstract

Fluorescence imaging in diffusive media is an emerging imaging modality for medical applications that uses injected fluorescent markers that bind to specific targets, e.g., carcinoma. The region of interest is illuminated with near-IR light and the emitted back fluorescence is analyzed to localize the fluorescence sources. To investigate a thick medium, as the fluorescence signal decreases with the light travel distance, any disturbing signal, such as biological tissues intrinsic fluorescence (called autofluorescence) is a limiting factor. Several specific markers may also be simultaneously injected to bind to different molecules, and one may want to isolate each specific fluorescent signal from the others. To remove the unwanted fluorescence contributions or separate different specific markers, a spectroscopic approach is explored. The nonnegative matrix factorization (NMF) is the blind positive source separation method we chose. We run an original regularized NMF algorithm we developed on experimental data, and successfully obtain separated in vivo fluorescence spectra.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Anne-Sophie Montcuquet, Lionel Hervé, Fabrice P. Navarro Y Garcia, Jean-Marc Dinten, and Jérôme I. Mars "Nonnegative matrix factorization: a blind spectra separation method for in vivo fluorescent optical imaging," Journal of Biomedical Optics 15(5), 056009 (1 September 2010). https://doi.org/10.1117/1.3491796

Published: 1 September 2010

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