Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells

Yan Li; Guiwen Wang; Hui-lu Yao; Junxian Liu; Yong-qing Li

doi:10.1117/1.3526357

1 November 2010 Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells

Yan Li, Guiwen Wang, Hui-lu Yao, Junxian Liu, Yong-qing Li

Author Affiliations +

Journal of Biomedical Optics, Vol. 15, Issue 6, 067008 (November 2010). https://doi.org/10.1117/1.3526357

Abstract

We report on development of dual-trap Raman tweezers for monitoring cellular dynamics and heterogeneity of interacting living cells suspended in a liquid medium. Dual-beam optical tweezers were combined with Raman spectroscopy, which allows capturing two cells that are in direct contact or closely separated by a few micrometers and simultaneously acquiring their Raman spectra with an imaging CCD spectrograph. As a demonstration, we recorded time-lapse Raman spectra of budding yeast cells held in dual traps for over 40 min to monitor the dynamic growth in a nutrient medium. We also monitored two germinating Bacillus spores after the initiation with L-alanine and observed their heterogeneity in the release of CaDPA under identical microenvironment.

©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Yan Li, Guiwen Wang, Hui-lu Yao, Junxian Liu, and Yong-qing Li "Dual-trap Raman tweezers for probing dynamics and heterogeneity of interacting microbial cells," Journal of Biomedical Optics 15(6), 067008 (1 November 2010). https://doi.org/10.1117/1.3526357

Published: 1 November 2010

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY