Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy

Seungrag Lee; Ji Yong Lee; Chang-Soo Park; Young Ran Kim; Joon Haeng Rhee; Dug Young Kim

doi:10.1117/1.3548882

1 March 2011 Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy

Seungrag Lee, Ji Yong Lee, Chang-Soo Park, Young Ran Kim, Joon Haeng Rhee, Dug Young Kim

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Journal of Biomedical Optics, Vol. 16, Issue 3, 036004 (March 2011). https://doi.org/10.1117/1.3548882

Abstract

We present the real-time quantitative analysis of Vibrio vulnificus-infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with V.vulnificus strains: wild type (MO6-24/O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificus infection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or β-hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner.

©(2011) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Seungrag Lee, Ji Yong Lee, Chang-Soo Park, Young Ran Kim, Joon Haeng Rhee, and Dug Young Kim "Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy," Journal of Biomedical Optics 16(3), 036004 (1 March 2011). https://doi.org/10.1117/1.3548882

Published: 1 March 2011

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