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21 May 2012 Metabolism of HeLa cells revealed through autofluorescence lifetime upon infection with enterohemorrhagic Escherichia coli
Tatyana Buryakina, Pin-Tzu Su, Wan-Jr Syu, C. Allen Chang, Hsui-Fang Fan, Fu-Jen Kao
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Journal of Biomedical Optics, Vol. 17, Issue 10, 101503 (May 2012). https://doi.org/10.1117/1.JBO.17.10.101503
Abstract
Fluorescence lifetime imaging microscopy (FLIM) is a sensitive technique in monitoring functional and conformational states of nicotinamide adenine dinucleotide reduced (NADH) and flavin adenine dinucleotide (FAD),main compounds participating in oxidative phosphorylation in cells. In this study, we have applied FLIM to characterize the metabolic changes in HeLa cells upon bacterial infection and made comparison with the results from the cells treated with staurosporine (STS), a well-known apoptosis inducer. The evolving of NADH's average autofluorescence lifetime during the 3 h after infection with enterohemorragic Escherichia coli (EHEC) or STS treatment has been observed. The ratio of the short and the long lifetime components' relative contributions of NADH increases with time, a fact indicating cellular metabolic activity, such as a decrease of oxidative phosphorylation over the course of infection, while opposite dynamics is observed in FAD. Being associated with mitochondria, FAD lifetimes and redox ratio could indicate heterogeneous mitochondrial function, microenvironment with bacterial infection, and further pathway to cell death. The redox ratios for both EHEC-infected and STS-treated HeLa cells have been observed and these observations also indicate possible apoptosis induced by bacterial infection.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Tatyana Buryakina, Pin-Tzu Su, Wan-Jr Syu, C. Allen Chang, Hsui-Fang Fan, and Fu-Jen Kao "Metabolism of HeLa cells revealed through autofluorescence lifetime upon infection with enterohemorrhagic Escherichia coli," Journal of Biomedical Optics 17(10), 101503 (21 May 2012). https://doi.org/10.1117/1.JBO.17.10.101503
Published: 21 May 2012
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KEYWORDS
Cell death
Luminescence
Fluorescence lifetime imaging
Mode conditioning cables
Picosecond phenomena
Proteins
Bacteria
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