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4 October 2012 Real-time study of shape and thermal fluctuations in the echinocyte transformation of human erythrocytes using defocusing microscopy
Sebastian Etcheverry, Maria Jose Gallardo, Pablo Solano, Mario Suwalsky, Oscar N. Mesquita, Carlos Saavedra
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Journal of Biomedical Optics, Vol. 17, Issue 10, 106013 (October 2012). https://doi.org/10.1117/1.JBO.17.10.106013
Abstract
We present a real-time method to measure the amplitude of thermal fluctuations in biological membranes by means of a new treatment of the defocusing microscopy (DM) optical technique. This approach was also applied to study the deformation of human erythrocytes to its echinocyte structure. This was carried out by making three-dimensional shape reconstructions of the cell and measuring the thermal fluctuations of its membrane, as the cell is exposed to the anti-inflammatory drug naproxen and as it recovers its original shape, when it is subsequently cleansed of the drug. The results showed biomechanical changes in the membrane even at low naproxen concentration (0.2 mM). Also, we found that when the cell recovered its original shape, the membrane properties were different compared to the nondrugged initial erythrocyte, indicating that the drug administration-recovery process is not completely reversible.
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Sebastian Etcheverry, Maria Jose Gallardo, Pablo Solano, Mario Suwalsky, Oscar N. Mesquita, and Carlos Saavedra "Real-time study of shape and thermal fluctuations in the echinocyte transformation of human erythrocytes using defocusing microscopy," Journal of Biomedical Optics 17(10), 106013 (4 October 2012). https://doi.org/10.1117/1.JBO.17.10.106013
Published: 4 October 2012
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KEYWORDS
Microscopy
Interfaces
Microscopes
Biomedical optics
Error analysis
3D image processing
3D image reconstruction
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