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5 June 2012 Comparative evaluation of differential laser-induced perturbation spectroscopy as a technique to discriminate emerging skin pathology
Raymond T. Kozikowski III, Sarah E. Smith, Jennifer A. Lee, William L. Castleman, Brian S. Sorg, David W. Hahn
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Journal of Biomedical Optics, Vol. 17, Issue 6, 067002 (June 2012). https://doi.org/10.1117/1.JBO.17.6.067002
Abstract
Fluorescence spectroscopy has been widely investigated as a technique for identifying pathological tissue; however, unrelated subject-to-subject variations in spectra complicate data analysis and interpretation. We describe and evaluate a new biosensing technique, differential laser-induced perturbation spectroscopy (DLIPS), based on deep ultraviolet (UV) photochemical perturbation in combination with difference spectroscopy. This technique combines sequential fluorescence probing (pre- and post-perturbation) with sub-ablative UV perturbation and difference spectroscopy to provide a new spectral dimension, facilitating two improvements over fluorescence spectroscopy. First, the differential technique eliminates significant variations in absolute fluorescence response within subject populations. Second, UV perturbations alter the extracellular matrix (ECM), directly coupling the DLIPS response to the biological structure. Improved biosensing with DLIPS is demonstrated in vivo in a murine model of chemically induced skin lesion development. Component loading analysis of the data indicates that the DLIPS technique couples to structural proteins in the ECM. Analysis of variance shows that DLIPS has a significant response to emerging pathology as opposed to other population differences. An optimal likelihood ratio classifier for the DLIPS dataset shows that this technique holds promise for improved diagnosis of epithelial pathology. Results further indicate that DLIPS may improve diagnosis of tissue by augmenting fluorescence spectra (i.e. orthogonal sensing).
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2012/$25.00 © 2012 SPIE
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Raymond T. Kozikowski III, Sarah E. Smith, Jennifer A. Lee, William L. Castleman, Brian S. Sorg, and David W. Hahn "Comparative evaluation of differential laser-induced perturbation spectroscopy as a technique to discriminate emerging skin pathology," Journal of Biomedical Optics 17(6), 067002 (5 June 2012). https://doi.org/10.1117/1.JBO.17.6.067002
Received: 4 February 2012; Accepted: 5 April 2012; Published: 5 June 2012
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KEYWORDS
Luminescence
Pathology
Skin
Laser induced fluorescence
Fluorescence spectroscopy
Laser spectroscopy
Spectroscopy
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