Surface plasmon-based infrared spectroscopy for cell biosensing

Victor Yashunsky; Vladislav G. Lirtsman; Alexander Zilbershtein; Michael Golosovsky; Dan Davidov; Amir Bein; Betty Schwartz; Benjamin Aroeti

doi:10.1117/1.JBO.17.8.081409

14 June 2012 Surface plasmon-based infrared spectroscopy for cell biosensing

Victor Yashunsky, Vladislav G. Lirtsman, Alexander Zilbershtein, Michael Golosovsky, Dan Davidov, Amir Bein, Betty Schwartz, Benjamin Aroeti

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Journal of Biomedical Optics, Vol. 17, Issue 8, 081409 (June 2012). https://doi.org/10.1117/1.JBO.17.8.081409

Abstract

Cell morphology is often used as a valuable indicator of the physical condition and general status of living cells. We demonstrate a noninvasive method for morphological characterization of adherent cells. We measure infrared reflectivity spectrum at oblique angle from living cells cultured on thin Au film, and utilize the unique properties of the confined infrared waves (i.e., surface plasmon and guided modes) traveling inside the cell layer. The propagation of these waves strongly depends on cell morphology and connectivity. By tracking the resonant wavelength and attenuation of the surface plasmon and guided modes we measure the kinetics of various cellular processes such as (i) cell attachment and spreading on different substrata, (ii) modulation of the outer cell membrane with chlorpromazine, and (iii) formation of intercellular junctions associated with progressive cell polarization. Our method enables monitoring of submicron variations in cell layer morphology in real-time, and in the label-free manner.

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Victor Yashunsky, Vladislav G. Lirtsman, Alexander Zilbershtein, Michael Golosovsky, Dan Davidov, Amir Bein, Betty Schwartz, and Benjamin Aroeti "Surface plasmon-based infrared spectroscopy for cell biosensing," Journal of Biomedical Optics 17(8), 081409 (14 June 2012). https://doi.org/10.1117/1.JBO.17.8.081409

Published: 14 June 2012

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