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24 September 2014 Real-time absorption reduced surface fluorescence imaging
Bin Yang, James W. Tunnell
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Journal of Biomedical Optics, Vol. 19, Issue 9, 090505 (September 2014). https://doi.org/10.1117/1.JBO.19.9.090505
Abstract
We introduce a technique that limits absorption effects in fluorescence imaging and does not require extensive imaging processing, thus allowing for video rate imaging. The absorption minimization is achieved using spatial frequency domain imaging at a single high spatial frequency with standard three-phase demodulation. At a spatial frequency f=0.5  mm−1, we demonstrated in both in-vitro phantoms and ex-vivo tissue that the absorption can be significantly reduced. In the real-time implementation, we achieved a video rate of 19  frames/s. This technique has potential in cancer visualization and tumor margin detection.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Bin Yang and James W. Tunnell "Real-time absorption reduced surface fluorescence imaging," Journal of Biomedical Optics 19(9), 090505 (24 September 2014). https://doi.org/10.1117/1.JBO.19.9.090505
Published: 24 September 2014
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Cited by 11 scholarly publications and 1 patent.
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KEYWORDS
Luminescence
Absorption
Spatial frequencies
Tissues
Image processing
Video
Tumors
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