Vessel packaging effect in laser speckle contrast imaging and laser Doppler imaging

Ingemar Fredriksson; Marcus Larsson

doi:10.1117/1.JBO.22.10.106005

10 October 2017 Vessel packaging effect in laser speckle contrast imaging and laser Doppler imaging

Ingemar Fredriksson, Marcus Larsson

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Journal of Biomedical Optics, Vol. 22, Issue 10, 106005 (October 2017). https://doi.org/10.1117/1.JBO.22.10.106005

Abstract

Laser speckle-based techniques are frequently used to assess microcirculatory blood flow. Perfusion estimates are calculated either by analyzing the speckle fluctuations over time as in laser Doppler flowmetry (LDF), or by analyzing the speckle contrast as in laser speckle contrast imaging (LSCI). The perfusion estimates depend on the amount of blood and its speed distribution. However, the perfusion estimates are commonly given in arbitrary units as they are nonlinear and depend on the magnitude and the spatial distribution of the optical properties in the tissue under investigation. We describe how the spatial confinement of blood to vessels, called the vessel packaging effect, can be modeled in LDF and LSCI, which affect the Doppler power spectra and speckle contrast, and the underlying bio-optical mechanisms for these effects. As an example, the perfusion estimate is reduced by 25% for LDF and often more than 50% for LSCI when blood is located in vessels with an average diameter of 40 μm, instead of being homogeneously distributed within the tissue. This significant effect can be compensated for only with knowledge of the average diameter of the vessels in the tissue.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Ingemar Fredriksson and Marcus Larsson "Vessel packaging effect in laser speckle contrast imaging and laser Doppler imaging," Journal of Biomedical Optics 22(10), 106005 (10 October 2017). https://doi.org/10.1117/1.JBO.22.10.106005

Received: 30 June 2017; Accepted: 12 September 2017; Published: 10 October 2017

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