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21 September 2017 Optical demodulation system for digitally encoded suspension array in fluoroimmunoassay
Qinghua He, Dongmei Li, Yonghong He, Tian Guan, Yilong Zhang, Zhiyuan Shen, Xuejing Chen, Siyu Liu, Bangrong Lu, Yanhong Ji
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Journal of Biomedical Optics, Vol. 22, Issue 9, 097003 (September 2017). https://doi.org/10.1117/1.JBO.22.9.097003
Abstract
A laser-induced breakdown spectroscopy and fluorescence spectroscopy-coupled optical system is reported to demodulate digitally encoded suspension array in fluoroimmunoassay. It takes advantage of the plasma emissions of assembled elemental materials to digitally decode the suspension array, providing a more stable and accurate recognition to target biomolecules. By separating the decoding procedure of suspension array and adsorption quantity calculation of biomolecules into two independent channels, the cross talk between decoding and label signals in traditional methods had been successfully avoided, which promoted the accuracy of both processes and realized more sensitive quantitative detection of target biomolecules. We carried a multiplexed detection of several types of anti-IgG to verify the quantitative analysis performance of the system. A limit of detection of 1.48×10−10  M was achieved, demonstrating the detection sensitivity of the optical demodulation system.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Qinghua He, Dongmei Li, Yonghong He, Tian Guan, Yilong Zhang, Zhiyuan Shen, Xuejing Chen, Siyu Liu, Bangrong Lu, and Yanhong Ji "Optical demodulation system for digitally encoded suspension array in fluoroimmunoassay," Journal of Biomedical Optics 22(9), 097003 (21 September 2017). https://doi.org/10.1117/1.JBO.22.9.097003
Received: 4 May 2017; Accepted: 5 September 2017; Published: 21 September 2017
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KEYWORDS
Luminescence
Demodulation
Laser induced breakdown spectroscopy
Plasma
Computer programming
Multiplexing
Pulsed laser operation
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