Scattering angle resolved optical coherence tomography measures morphological changes in Bacillus subtilis colonies

Vikram Barauah; Shyon Parsa; Naail R. Chowdhury; Thomas E. Milner; Henry Grady Rylander III

doi:10.1117/1.JBO.27.12.126004

30 December 2022 Scattering angle resolved optical coherence tomography measures morphological changes in Bacillus subtilis colonies

Vikram Barauah, Shyon Parsa, Naail R. Chowdhury, Thomas E. Milner, Henry Grady Rylander III

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Journal of Biomedical Optics, Vol. 27, Issue 12, 126004 (December 2022). https://doi.org/10.1117/1.JBO.27.12.126004

Abstract

Significance

An unmet need is recognized for early detection and diagnosis of neurological diseases. Many psychological markers emerge years after disease onset. Mitochondrial dysfunction and corresponding neurodegeneration occur before onset of large-scale cell and tissue pathology. Early detection of subcellular morphology changes could serve as a beacon for early detection of neurological diseases. This study is on bacterial colonies, Bacillus subtilis, which are similar in size to mitochondria.

Aim

This study investigates whether morphological changes can be detected in Bacillus subtilis using scattering angle resolved optical coherence tomography (SAR-OCT).

Approach

The SAR-OCT was applied to detect scattering angle distribution changes in Bacillus subtilis. The rod-to-coccus shape transition of the bacteria was imaged, and the backscattering angle was analyzed by recording the distribution of the ratio of low- to medium angle scattering (L/M ratio). Bacillus orientation at different locations in colonies was analytically modeled and compared with SAR-OCT results.

Results

Significant differences in the distribution of backscattering angle were observed in Bacillus subtilis transitioning from rod-to-coccus shapes. In Bacillus subtilis, the C-parameter of the Burr distribution of the SAR-OCT-derived L/M ratio was significantly smaller in coccus compared with rod-shaped bacteria. SAR-OCT-derived L/M ratio varied with bacterial position in the colony and is consistent with predicted orientations from previous studies.

Conclusions

Study results support the potential of utilizing SAR-OCT to detect bacterial morphological changes.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Vikram Barauah, Shyon Parsa, Naail R. Chowdhury, Thomas E. Milner, and Henry Grady Rylander III "Scattering angle resolved optical coherence tomography measures morphological changes in Bacillus subtilis colonies," Journal of Biomedical Optics 27(12), 126004 (30 December 2022). https://doi.org/10.1117/1.JBO.27.12.126004

Received: 20 April 2022; Accepted: 22 November 2022; Published: 30 December 2022

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KEYWORDS

Bacteria

Optical coherence tomography

Scattering

Mitochondria

Light scattering

Backscatter

Neurological disorders

Significance

Aim

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