Nonlinear matching measure for the analysis of on-off type DNA microarray images

Munho Ryu; Jong Dae Kim; Byoung Goo Min; Myung-Geol Pang; Jongwon Kim

doi:10.1117/1.1691026

1 May 2004 Nonlinear matching measure for the analysis of on-off type DNA microarray images

Munho Ryu, Jong Dae Kim, Byoung Goo Min, Myung-Geol Pang, Jongwon Kim

Author Affiliations +

Journal of Biomedical Optics, Vol. 9, Issue 3, (May 2004). https://doi.org/10.1117/1.1691026

Abstract

We propose a new nonlinear matching measure for automatic analysis of the on-off type DNA microarray images in which the hybridized spots are detected by a template-matching method. The proposed measure is obtained by binary thresholding over the entire template region and taking the number of white pixels inside the spotted area. This measure is compared with the normalized covariance method in terms of classifying the ability to successfully locate markers. The proposed measure was evaluated for scanned images of human papillomavirus (HPV) DNA microarrays where locating markers is a critical issue because of the small number of spots. The targeting spots of HPV DNA chips are designed for genotyping twenty-two types of the human papillomavirus. The proposed measure is proven to give a more discriminative response, reducing the missed cases of successful marker location. The locating accuracy of the proposed method is also shown to have the same performance as that of the normalized covariance.

©(2004) Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation Download Citation

Munho Ryu, Jong Dae Kim, Byoung Goo Min, Myung-Geol Pang, and Jongwon Kim "Nonlinear matching measure for the analysis of on-off type DNA microarray images," Journal of Biomedical Optics 9(3), (1 May 2004). https://doi.org/10.1117/1.1691026

Published: 1 May 2004

ACCESS THE FULL ARTICLE

JOURNAL ARTICLE
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

CITATIONS

Cited by 1 scholarly publication.

Explore citations on Lens.org

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Failure analysis

Binary data

Analytical research

Biomedical engineering

Communication engineering

Image processing

Medical research

Show All Keywords

RELATED CONTENT

Text/graphics separation for technical papers
Proceedings of SPIE (March 30 1995)

Maximizing reading: pattern analysis to describe points of gaze
Proceedings of SPIE (August 25 2006)

The Use Of Digital Processors In Image Analysis Systems
Proceedings of SPIE (December 29 1978)

Novel block segmentation and processing for Chinese-English document
Proceedings of SPIE (November 01 1991)

Integration Of Imagery And Cartographic Data Through A Common Map...
Proceedings of SPIE (March 17 1983)

Dynamic threshold using polynomial surface regression with application to the...
Proceedings of SPIE (March 28 2005)

Knowledge-based image processing for on-off type DNA microarray
Proceedings of SPIE (June 05 2002)

Subscribe to Digital Library

Receive Erratum Email Alert

Show All Keywords

Keywords/Phrases

Search In:

Publication Years