CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > Neurophoton. > Volume 2 > Issue 4 > Article
Open Access
9 November 2015 Ultramicroscopy: development and outlook
Hans-Ulrich Dodt, Saiedeh Saghafi, Klaus Becker, Nina Jährling, Axel Niendorf, Christian Hahn, Marko Pende, Martina Wanis
Author Affiliations +
Neurophotonics, Vol. 2, Issue 4, 041407 (November 2015). https://doi.org/10.1117/1.NPh.2.4.041407
Abstract
We present an overview of the ultramicroscopy technique we developed. Starting from developments 100 years ago, we designed a light sheet microscope and a chemical clearing to image complete mouse brains. Fluorescence of green fluorescent protein (GFP)-labeled neurons in mouse brains could be preserved with our 3DISCO clearing and high-resolution three-dimensional (3-D) recordings were obtained. Ultramicroscopy was also used to image whole mouse embryos and flies. We improved the optical sectioning of our light sheet microscope by generating longer and thinner light sheets with aspheric optics. To obtain high-resolution images, we corrected available air microscope objectives for clearing solutions with high refractive index. We discuss how eventually super resolution could be realized in light sheet microscopy by applying stimulated emission depletion technology. Also the imaging of brain function by recording of mouse brains expressing cfos-GFP is discussed. Finally, we show the first 3-D recordings of human breast cancer with light sheet microscopy as application in medical diagnostics.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Hans-Ulrich Dodt, Saiedeh Saghafi, Klaus Becker, Nina Jährling, Axel Niendorf, Christian Hahn, Marko Pende, and Martina Wanis "Ultramicroscopy: development and outlook," Neurophotonics 2(4), 041407 (9 November 2015). https://doi.org/10.1117/1.NPh.2.4.041407
Published: 9 November 2015
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 22 scholarly publications and 4 patents.
Advertisement
Advertisement
KEYWORDS
Brain
Visualization
Green fluorescent protein
Neurons
Neuroimaging
Luminescence
Microscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top