Heterogeneity plays an important role in medicine and biology, which can be investigated by exploiting single cell analysis (SCA). Among SCA methods, imaging cytometry allows the analysis of individual 2D and 3D spatial features. Here we present a femtosecond laser fabricated optofluidic automated platform encompassing a thermo-optic phase shifter, cylindrical lenses and a microfluidic network to generate and shift a dual-color patterned light sheet within a microchannel where the samples of interest flow. The device can be used as add-on and can provide an acquisition rate of about 1 cell/second, or subnuclear resolution at the single cell level.
Optical fibre consisting of a pure silicon core in silica cladding combines the advantageous properties of silicon waveguides with the convenience of optical fibre. However, the optical quality of these fibres is highly dependent on the crystalline structure and the purity of the silicon. The fabrication of these fibres requires engineering of the thermal gradients during the drawing process to ensure optimal crystallisation of the silicon. Here, we investigated the effects of draw speed and analyse the induced stresses at multiple stages in the fabrication process. The thermal exposure of the silicon while in contact with a silica cladding was found to increase the optical losses. This was attributed to the diffusion of impurities from the silica cladding into the silicon core.
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