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17 March 2023 Dual-color optogenetic tool enables heart pacing and restorable heart arrest in D.melanogaster
Elena Gracheva, Fei Wang, Yuxuan Wang, Jiantao Zhu, Matthew Fishman, Abigail Matt, Hongwu Liang, Chao Zhou
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Proceedings Volume PC12366, Optogenetics and Optical Manipulation 2023; PC1236608 (2023) https://doi.org/10.1117/12.2650935
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We have developed dual-color Drosophila melanogaster (fruit fly) optogenetic system, based on transgenic line containing two opsins, ChR2 and NpHR2.0, and a customized Optical Coherence Microscopy (OCM) hardware. The initial experiments demonstrated feasibility of increasing the heart rate following the designed blue light pulses and inducing the restorable heart arrest caused by prolonged red light illumination in the same animal. The heart function control was achieved non-invasively. We have optimized the OCT system parameters to minimize the detrimental effects on live animals to ensure longitudinal studies on Drosophila models of human heart arrhythmia disorders.
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Elena Gracheva, Fei Wang, Yuxuan Wang, Jiantao Zhu, Matthew Fishman, Abigail Matt, Hongwu Liang, and Chao Zhou "Dual-color optogenetic tool enables heart pacing and restorable heart arrest in D.melanogaster", Proc. SPIE PC12366, Optogenetics and Optical Manipulation 2023, PC1236608 (17 March 2023); https://doi.org/10.1117/12.2650935
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KEYWORDS
Heart
Optogenetics
Coherence imaging
Imaging systems
Animal model studies
Genetics
Optical coherence microscopy
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