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13 March 2024 Visualization of drug uptake and its cellular response by Raman microscopy
Katsumasa Fujita
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Proceedings Volume PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII; PC1282105 (2024) https://doi.org/10.1117/12.3009021
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
We applied Raman microscopy to observe the response of intracellular molecules after drug administration. We found that the dynamics change in heme protein redox states and distributions can be imaged without labelling. We also applied alkyne-tag Raman imaging to detect the uptake of drugs and quantitatively compare the efficiency under different drug administration conditions.
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Katsumasa Fujita "Visualization of drug uptake and its cellular response by Raman microscopy", Proc. SPIE PC12821, Visualizing and Quantifying Drug Distribution in Tissue VIII, PC1282105 (13 March 2024); https://doi.org/10.1117/12.3009021
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KEYWORDS
Raman spectroscopy
Optical microscopy
Visualization
Mitochondria
Molecules
Cell death
Live cell imaging
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