Analysis of microorganisms, chlorinated hydrocarbons and hyaluronic acid gel using Raman based optofluidic techniques and SERS

Ota Samek; Zdenek Pilat; Silvie Bernatova; Jan Jezek; Tereza Klementova; Martin Kizovsky; Pavel Zemanek; Katarina Rebrosova; Filip Ruzicka

doi:10.1117/12.2508425

7 March 2019 Analysis of microorganisms, chlorinated hydrocarbons and hyaluronic acid gel using Raman based optofluidic techniques and SERS

Ota Samek, Zdenek Pilat, Silvie Bernatova, Jan Jezek, Tereza Klementova, Martin Kizovsky, Pavel Zemanek, Katarina Rebrosova, Filip Ruzicka

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Proceedings Volume 10894, Plasmonics in Biology and Medicine XVI; 108940D (2019) https://doi.org/10.1117/12.2508425
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We report on the development of a set of Raman based techniques to monitor a large variety of biological and chemical analytes, such as various microorganisms, gels of hyaluronic acid and selected halogenated hydrocarbons using Raman spectroscopy, Raman tweezers and surface-enhanced Raman spectroscopy (SERS). We analyzed individual microbial cells with Raman tweezers to provide solutions for fast and label-free identification of specific bacterial or yeast species. We designed an optofluidic SERS platform for quantification of sub-millimolar concentrations of halogenated environmental pollutants such as 1,2,3-trichloropropane and chloroform. We also examined the gel structure of hyaluronic acid by Raman spectroscopy.

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Ota Samek, Zdenek Pilat, Silvie Bernatova, Jan Jezek, Tereza Klementova, Martin Kizovsky, Pavel Zemanek, Katarina Rebrosova, and Filip Ruzicka "Analysis of microorganisms, chlorinated hydrocarbons and hyaluronic acid gel using Raman based optofluidic techniques and SERS ", Proc. SPIE 10894, Plasmonics in Biology and Medicine XVI, 108940D (7 March 2019); https://doi.org/10.1117/12.2508425

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