3D morphology of melanoma cells using digital holographic interferometry

Natalith Palacios-Ortega; Fernando Mendoza Santoyo; J. Mauricio Flores Moreno; María del Socorro Hernández-Montes; Manuel H. De la Torre Ibarra; Germán Plascencia

doi:10.1117/12.2529000

3 September 2019 3D morphology of melanoma cells using digital holographic interferometry

Natalith Palacios-Ortega, Fernando Mendoza Santoyo, J. Mauricio Flores Moreno, María del Socorro Hernández-Montes, Manuel H. De la Torre Ibarra, Germán Plascencia

Author Affiliations +

Proceedings Volume 11102, Applied Optical Metrology III; 111020H (2019) https://doi.org/10.1117/12.2529000
Event: SPIE Optical Engineering + Applications, 2019, San Diego, California, United States

Abstract

Depending on its stage skin cancer can be diagnosed through the so called ABCDE rule, which is mainly qualitative and noninvasive; besides this, there exists a technique that uses a dermatoscope to analyze the damaged area considered as noninvasive. On the other hand, the invasive methods mainly use sample-biopsies extracted from the affected area which are usually analyzed with a microscope or other imaging techniques. Digital holographic interferometry (DHI) has shown to be a powerful technique to obtain 3D information of a sample, and in these times, as many other optical metrology techniques, it has been widely applied to study biologic samples. In this research work, we present some initial 3D morphology measurements of melanoma cells made with a DHI setup implemented in a microscope type arrangement so a magnified image of the sample is studied. The morphology data depict the actual 3D contour map of the cell nucleus and represents a further advancement in the application of DHI to accurately study and measure the structure of cells.

Citation Download Citation

Natalith Palacios-Ortega, Fernando Mendoza Santoyo, J. Mauricio Flores Moreno, María del Socorro Hernández-Montes, Manuel H. De la Torre Ibarra, and Germán Plascencia "3D morphology of melanoma cells using digital holographic interferometry", Proc. SPIE 11102, Applied Optical Metrology III, 111020H (3 September 2019); https://doi.org/10.1117/12.2529000

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