Characterizing long lived intralipid-infused tissue phantoms scattering using imaging sensors

Glenn H. Chapman; Sean Paulsen; Yutian Zhang

doi:10.1117/12.2548689

25 February 2020 Characterizing long lived intralipid-infused tissue phantoms scattering using imaging sensors

Glenn H. Chapman, Sean Paulsen, Yutian Zhang

Proceedings Volume 11238, Optical Interactions with Tissue and Cells XXXI; 112380S (2020) https://doi.org/10.1117/12.2548689
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Since regular biological tissue’s optical characteristics are unstable building long lived test phantoms that simulate their scattering characteristics are needed for testing optical methods of imaging through tissue. Our ongoing work developed a methodology to build long term stable phantoms with lifetimes >6 years which can be tuned to maintaining optical characteristics mimicing skin characteristics and a rapid technique to measure the scattering coefficients μ_s and anisotropy factor g. Our test phantoms employ intralipid-infused agar layers 1 to 8 mm thick. Agra can take a wide range of intralipid concentrations enabling the building phantoms of a large range of scattering parameters with typical values of μ_s=20cm^-1, g=0.95. Encapsulating the intralipid-infused agar within a clear polymer has proved to stabilize these for long lifetimes and allows creation of varying thicknesses, scattering characteristics and shapes. We developed a rapid technique where the light from a laser beam passing perpendicular through the test phantom is captured using a 36×24mm digital camera sensor. This gathers ~6×10⁶ measurements over a ±12° range, giving ~20,000 points each at 2300 angular bins of 0.005° . A Matlab program identifies the scattering center and data points for each angular position. Using a HenyeyGreenstein two-term model a nonlinear curve fitting extracts pairs of HG weighing factors, µs and g parameters. Fit results show extremely high statistical significance with exceedingly small deviation from the HG model for multiple wavelengths (currently 533, 632, 670 nm lasers and a supercontinuum laser at 650, 700,750 nm) for several test phantoms.

Conference Presentation

Citation Download Citation

Glenn H. Chapman, Sean Paulsen, and Yutian Zhang "Characterizing long lived intralipid-infused tissue phantoms scattering using imaging sensors", Proc. SPIE 11238, Optical Interactions with Tissue and Cells XXXI, 112380S (25 February 2020); https://doi.org/10.1117/12.2548689

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available