Competitive binding of gold nanospheres and nanorods on DNA origami substrates

Divita Mathur; William P. Klein; Hieu Bui; Eunkeu Oh; Jawad Naciri; Jake Fontana; Sebastián A. Díaz; Igor L. Medintz

doi:10.1117/12.2545953

21 February 2020 Competitive binding of gold nanospheres and nanorods on DNA origami substrates

Divita Mathur, William P. Klein, Hieu Bui, Eunkeu Oh, Jawad Naciri, Jake Fontana, Sebastián A. Díaz, Igor L. Medintz

Proceedings Volume 11255, Colloidal Nanoparticles for Biomedical Applications XV; 1125509 (2020) https://doi.org/10.1117/12.2545953
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

DNA-directed assembly of gold nanoparticles into precise two- and three-dimensional patterns has enabled bold advances in probing their optical properties such as the local enhancement in their surface plasmon resonance. DNA nanostructures synthesized using the principles of DNA origami have been programmed to contain unique capture sites for positioning metal nanoparticles in diverse geometries for applications in biosensing, therapy, and miniature electronics. However, to enable scalability beyond simple 2-3 nanoparticle architectures, it is important to understand the requirement for orthogonal capture sequences for attaching more than a single gold nanoparticle on a DNA nanostructure. In this work, we sought to assemble an angular gold nanorod-nanosphere-nanorod pattern on a DNA origami triangle with multiple capture sites utilizing a common capture sequence. Results indicate that gold nanospheres preferentially bound to all the capture sites on the DNA origami triangle and prevented attachment of gold nanorods. This suggests that requirement for orthogonal capture sites is correlated with the physical properties of the individual nanoparticle such as shape and size.

Citation Download Citation

Divita Mathur, William P. Klein, Hieu Bui, Eunkeu Oh, Jawad Naciri, Jake Fontana, Sebastián A. Díaz, and Igor L. Medintz "Competitive binding of gold nanospheres and nanorods on DNA origami substrates", Proc. SPIE 11255, Colloidal Nanoparticles for Biomedical Applications XV, 1125509 (21 February 2020); https://doi.org/10.1117/12.2545953

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available