FT-IR Microscopy Of Biomineralization At 20Âµ Spatial Resolution

Richard Mendelsohn; Alborz Hassankhani; Nancy Pleshko; Edward DiCarlo; Adele Boskey

doi:10.1117/12.969521

1 December 1989 FT-IR Microscopy Of Biomineralization At 20µ Spatial Resolution

Richard Mendelsohn, Alborz Hassankhani, Nancy Pleshko, Edward DiCarlo, Adele Boskey

Proceedings Volume 1145, 7th Intl Conf on Fourier Transform Spectroscopy; (1989) https://doi.org/10.1117/12.969521
Event: Seventh International Conference on Fourier and Computerized Infrared Spectroscopy, 1989, Fairfax, VA, United States

Abstract

FT-IR microscopy offers certain advantages for the study of normal and pathological biomineralizing tissue samples. These include the identification and quantitation of the mineral phase and the determination of the secondary structure of the protein constituents. A drawback of the method is difficulty in interpretation of spectra from samples containing unknown quantities of unknown constituents. We have circumvented this problem by studying the main components of mineralizing tissue, collagen (95% of the protein phase) and hydroxyapatite (main mineral constituent) whose strongest IR bands absorb in relatively clean spectral regions. Samples were prepared for IR microscopy by surgical removal of tissue, fixation in formalin or ethanol, embedding in PMMA or epoxy, and the microtoming of thin (5-8μ) sections. Bulk embedding agent was mostly removed with methyl acetate. The sample was squeezed between BaF2 windows for IR studies. Any remaining PMMA or epoxy was removed by spectral subtraction. To evaluate the utility of FT-IR microscopy for study of biomineralization, two systems were examined:

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Richard Mendelsohn, Alborz Hassankhani, Nancy Pleshko, Edward DiCarlo, and Adele Boskey "FT-IR Microscopy Of Biomineralization At 20µ Spatial Resolution", Proc. SPIE 1145, 7th Intl Conf on Fourier Transform Spectroscopy, (1 December 1989); https://doi.org/10.1117/12.969521

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KEYWORDS

Microscopy

Minerals

Tissues

Crystals

FT-IR spectroscopy

Fourier transforms

Proteins

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