Optical-sectioning super-resolution structured illumination microscopy for fast virtual pathology

Ivan Bozic; Jonathon Q. Brown

doi:10.1117/12.2578440

5 March 2021 Optical-sectioning super-resolution structured illumination microscopy for fast virtual pathology

Ivan Bozic, Jonathon Q. Brown

Proceedings Volume 11622, Multiscale Imaging and Spectroscopy II; 1162205 (2021) https://doi.org/10.1117/12.2578440
Event: SPIE BiOS, 2021, Online Only

Abstract

On-site pathology of the surface of resected tumors provides real-time assessments of surgical procedure and improve treatment outcomes. Tissue preparation is a major bottleneck of standard pathology procedures that can be overcome by virtual pathology imaging. Here, we use optical sectioning super-resolution structured illumination microscopy (OS-SR-SIM) with a low magnification objective. Low magnification objective provides a higher field of view, and super-resolution reconstruction retrieves lost resolution due to lower NA. We successfully resolved sub-resolution fluorescent beads in highly scattering media that were not resolvable with standard OS-SIM with an NA 0.45 objective. Human tissue testing is underway.

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Ivan Bozic and Jonathon Q. Brown "Optical-sectioning super-resolution structured illumination microscopy for fast virtual pathology", Proc. SPIE 11622, Multiscale Imaging and Spectroscopy II, 1162205 (5 March 2021); https://doi.org/10.1117/12.2578440

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KEYWORDS

Microscopy

Pathology

Super resolution

Tissue optics

Tissues

Image resolution

Natural surfaces

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