Comprehensive evaluation of ex vivo and in vivo tissues with ultraviolet photoacoustic remote sensing virtual histology and spectral-domain optical coherence tomography

Matthew T. Martell; Nathaniel J. M. Haven; Brendon S. Restall; Pradyumna Kedarisetti; Roger J. Zemp

doi:10.1117/12.2578954

5 March 2021 Comprehensive evaluation of ex vivo and in vivo tissues with ultraviolet photoacoustic remote sensing virtual histology and spectral-domain optical coherence tomography

Matthew T. Martell, Nathaniel J. M. Haven, Brendon S. Restall, Pradyumna Kedarisetti, Roger J. Zemp

Author Affiliations +

Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116422W (2021) https://doi.org/10.1117/12.2578954
Event: SPIE BiOS, 2021, Online Only

Abstract

Rapid, accurate intraoperative assessment of excised specimens and in vivo resection sites is a critical need in tissue-conserving cancer surgeries. We develop a multimodal imaging system integrating ultraviolet photoacoustic remote sensing (UV-PARS) microscopy with spectral-domain optical coherence tomography (SD-OCT) to provide comprehensive, co-registered information for identifying atypical tissue features. 1050-nm SD-OCT generates a widefield, depth-resolved view of subsurface tissue morphology, with 9-μm axial resolution. UV-PARS produces virtual histological C-scan images of cell nuclei, at 390-nm lateral resolution. Dual-contrast in vivo mouse imaging, and ex vivo imaging of breast and prostate specimens is demonstrated, and correlated with conventional H and E staining.

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Matthew T. Martell, Nathaniel J. M. Haven, Brendon S. Restall, Pradyumna Kedarisetti, and Roger J. Zemp "Comprehensive evaluation of ex vivo and in vivo tissues with ultraviolet photoacoustic remote sensing virtual histology and spectral-domain optical coherence tomography", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422W (5 March 2021); https://doi.org/10.1117/12.2578954

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