High speed super-resolution localization photoacoustic microscopy

Jongbeom Kim; Jin Young Kim; Yeonggeun Kim; Chulhong Kim

doi:10.1117/12.2578111

12 March 2021 High speed super-resolution localization photoacoustic microscopy

Jongbeom Kim, Jin Young Kim, Yeonggeun Kim, Chulhong Kim

Proceedings Volume 11654, High-Speed Biomedical Imaging and Spectroscopy VI; 1165409 (2021) https://doi.org/10.1117/12.2578111
Event: SPIE BiOS, 2021, Online Only

Abstract

Photoacoustic imaging is a promising biomedical imaging technology that can provide the biological information of animals and humans in vivo. Taking advantages of both optics and ultrasound simultaneously, the photoacoustic imaging has rich optical contrast and high ultrasonic resolution in deep tissues. Especially, the optical-resolution photoacoustic microscopy (OR-PAM), the major implementation of the photoacoustic imaging, achieves sharp spatial resolution with focused optical beams. However, developed OR-PAM systems are disadvantaged by limited temporal and/or spatial resolutions by hardware parts. Here, we introduce a high speed super-resolution localization OR-PAM that overcomes the limited resolutions. First, to improve a temporal resolution, we equipped a galvanometer scanner, which has been used in many optical microscopies. Previously, due to the vulnerability of the scanner to water, the PAM system using the scanner scanned only light, not ultrasound. However, our system overcame the weakness by immersing only a scanning part except to a galvanometer part, leading to scanning light and ultrasound simultaneously. Steering both laser beams and ultrasound waves with a scanner’s mirror immersed in the water, our system achieves a wide scanning range and a high signal-to-noise ratio as well as the B-mode imaging speed of 500 Hz. Furthermore, we acquired a super-resolved microvascular image in vivo using an agent-free localization imaging technology based on the fast scanning speed. These results show that our super-resolution high-speed OR-PAM can be used in a variety of fields, including neurology, oncology, and pathology.

Conference Presentation

Citation Download Citation

Jongbeom Kim, Jin Young Kim, Yeonggeun Kim, and Chulhong Kim "High speed super-resolution localization photoacoustic microscopy", Proc. SPIE 11654, High-Speed Biomedical Imaging and Spectroscopy VI, 1165409 (12 March 2021); https://doi.org/10.1117/12.2578111

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