3D microscopy in extreme conditions: in-flow or at high density

Yoav Shechtman; Elias Nehme; Boris Ferdman; Omer Adir; Racheli Gordon-Soffer; Reut Orange; Tal Naor; Yael Shalev-Ezra; Sarah Goldberg; Onit Alalouf; Daniel Freedman; Avi Schroeder; Tomer Michaeli; Lucien E. Weiss

doi:10.1117/12.2582466

5 March 2021 3D microscopy in extreme conditions: in-flow or at high density

Yoav Shechtman, Elias Nehme, Boris Ferdman, Omer Adir, Racheli Gordon-Soffer, Reut Orange, Tal Naor, Yael Shalev-Ezra, Sarah Goldberg, Onit Alalouf, Daniel Freedman, Avi Schroeder, Tomer Michaeli, Lucien E. Weiss

Author Affiliations +

Proceedings Volume 11654, High-Speed Biomedical Imaging and Spectroscopy VI; 116540R (2021) https://doi.org/10.1117/12.2582466
Event: SPIE BiOS, 2021, Online Only

Abstract

Attaining three-dimensional data at high throughput is a grand challenge in microscopy. I will discuss two recent contributions to 3D microscopy utilizing point-spread-function (PSF) engineering: 1.a new method that extends the capabilities of flow-based imaging to 3D co-localization microscopy, and 2. efficient and fast localization of dense molecules in volumetric single-molecule-localization microscopy (SMLM) using deep learning, termed DeepSTORM3D. Together, these methods enable the study of sub-cellular biological processes at unprecedented timescales and throughputs.

Conference Presentation

Citation Download Citation

Yoav Shechtman, Elias Nehme, Boris Ferdman, Omer Adir, Racheli Gordon-Soffer, Reut Orange, Tal Naor, Yael Shalev-Ezra, Sarah Goldberg, Onit Alalouf, Daniel Freedman, Avi Schroeder, Tomer Michaeli, and Lucien E. Weiss "3D microscopy in extreme conditions: in-flow or at high density", Proc. SPIE 11654, High-Speed Biomedical Imaging and Spectroscopy VI, 116540R (5 March 2021); https://doi.org/10.1117/12.2582466

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