Sensing of bacterial spores with 2D-IR spectroscopy

Barbara Procacci; Samantha H. Rutherford; Gregory M. Greetham; Michael Towrie; Anthony W. Parker; Camilla V. Robinson; Christopher R. Howle; Neil T. Hunt

doi:10.1117/12.2585894

12 April 2021 Sensing of bacterial spores with 2D-IR spectroscopy

Barbara Procacci, Samantha H. Rutherford, Gregory M. Greetham, Michael Towrie, Anthony W. Parker, Camilla V. Robinson, Christopher R. Howle, Neil T. Hunt

Author Affiliations +

Proceedings Volume 11749, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXII; 117490C (2021) https://doi.org/10.1117/12.2585894
Event: SPIE Defense + Commercial Sensing, 2021, Online Only

Abstract

Ultrafast 2D-IR spectroscopy has proved to be a powerful analytical tool for the detection and differentiation of Bacillus spores as dry films on surfaces. Here, we expand on these findings by employing 2D-IR spectroscopy to study spores from B. atrophaeus (BG) in aqueous solution. Specific vibrational modes attributable to the calcium dipicolinate trihydrate biomarker for spore formation were observed alongside distinctive off-diagonal spectral features that can be used to differentiate spores from different Bacillus species, indicating that 2D-IR has potential for use as a sensing platform with both solid and liquid phase samples. The ability of 2D-IR to enhance the protein amide I band relative to the overlapping water bending vibration was exploited to compare the nature of the protein component of spores to that of solution phase protein molecules. The vibrational lifetime for the amide I band of the BG spore in H₂O was 1.4 ± 0.1 ps, longer than those reported for the proteins in H₂O solution. The nature of a band at 1710 cm^-1 was also investigated. Collectively these results show the potential advantages of 2D-IR spectroscopy, with successful detection and classification of spores under different conditions being based on detailed molecular understanding of the spore state.

Conference Presentation

Citation Download Citation

Barbara Procacci, Samantha H. Rutherford, Gregory M. Greetham, Michael Towrie, Anthony W. Parker, Camilla V. Robinson, Christopher R. Howle, and Neil T. Hunt "Sensing of bacterial spores with 2D-IR spectroscopy", Proc. SPIE 11749, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XXII, 117490C (12 April 2021); https://doi.org/10.1117/12.2585894

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