Viral detection and quantification in a digital droplet microfluidic lab-in-a-fiber device

Helen E. Parker; Sanghamitra Sengupta; Achar V. Harish; Ruben R. G. Soares; Haakan N. Joensson; Walter Margulis; Aman Russom; Fredrik Laurell

doi:10.1117/12.2590850

18 April 2021 Viral detection and quantification in a digital droplet microfluidic lab-in-a-fiber device

Helen E. Parker, Sanghamitra Sengupta, Achar V. Harish, Ruben R. G. Soares, Haakan N. Joensson, Walter Margulis, Aman Russom, Fredrik Laurell

Author Affiliations +

Proceedings Volume 11773, Micro-structured and Specialty Optical Fibres VII; 117730Y (2021) https://doi.org/10.1117/12.2590850
Event: SPIE Optics + Optoelectronics, 2021, Online Only

Abstract

In this work, we present the design and fabrication of a fiber device that performs digital droplet microfluidics for molecular diagnostics. A variety of fibers and capillaries were used to build three connected modules dedicated to droplet generation, incubation, and fluorescence detection which enables a uniaxial arrangement. This is in contrast to the traditional 2-dimensional lab-on-a-chip architecture. We characterize our fiber device using a fluorescein dilution series. Our observed detection limit is on the order of 10 nM fluorescein. We demonstrate our all-fiber device for the fluorescence readout after loop-mediated isothermal amplification (LAMP) of synthetic SARS-CoV-2. Our results suggest that this fiber device can successfully distinguish between positive and negative samples in molecular diagnostics. We propose that our fiber device offers benefits over microfluidic chip techniques such as easier optical integration, much simpler sample loading, and faster diagnosis with high specificity and sensitivity.

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Citation Download Citation

Helen E. Parker, Sanghamitra Sengupta, Achar V. Harish, Ruben R. G. Soares, Haakan N. Joensson, Walter Margulis, Aman Russom, and Fredrik Laurell "Viral detection and quantification in a digital droplet microfluidic lab-in-a-fiber device", Proc. SPIE 11773, Micro-structured and Specialty Optical Fibres VII, 117730Y (18 April 2021); https://doi.org/10.1117/12.2590850

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