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Vascular-targeted photodynamic therapy (V-PDT) offers great promise as a treatment modality for vascular-related diseases. The injury of targeted blood vessels correlates to the singlet oxygen generation, which is affected by dosimetric parameters, including photosensitizer concentration, hemoglobin oxygenation concentration, and blood flow velocity. In this study, we developed an optical imaging system that combining hyperspectral imaging (HSI), dual-wavelength reflection imaging (DWRI) (λ1 = 500 nm and λ2 = 660 nm) and laser speckle imaging (LSI). The capability for monitoring dosimetric parameters has been demonstrated for in vivo imaging of hemoporfin-mediated V-PDT in a dorsal skinfold window chamber model. The HSI allows for simultaneously monitoring the changes of photosensitizer concentration and vasoconstriction of blood vessels, while the DWRI and LSI were used to measure hemoglobin oxygenation concentration and blood flow velocity, respectively. This study suggests that our home-built optical imaging system holds the potential for assessing the V-PDT efficiency in vivo and optimizing the treatment protocol.
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