Single objective tilted lightsheet for three-dimensional localization microscopy

Shih-Te Hung; Jelmer Cnossen; Daniel Fan; Carlas S. Smith

doi:10.1117/12.2615803

7 December 2021 Single objective tilted lightsheet for three-dimensional localization microscopy

Shih-Te Hung, Jelmer Cnossen, Daniel Fan, Carlas S. Smith

Proceedings Volume 11922, Advances in Microscopic Imaging III; 119221A (2021) https://doi.org/10.1117/12.2615803
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

Optical sectioning technologies achieve high precision localization by reducing the background photon count. We use tilted light-sheet microscopy to achieve optical sectioning in localization microscopy, enabling thick sample observation and low background photon count images. A deformable mirror was incorporated to generate a tetrapod point spread function (PSF), enabling high resolution 3D localization. DNA-PAINT was imaged with 15 nm transverse and 60 nm axial resolution.

Citation Download Citation

Shih-Te Hung, Jelmer Cnossen, Daniel Fan, and Carlas S. Smith "Single objective tilted lightsheet for three-dimensional localization microscopy", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119221A (7 December 2021); https://doi.org/10.1117/12.2615803

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