Simultaneous three-dimensional tracking of a mother colony and a daughter colony of a moving Volvox by parallel phase-shifting digital holographic microscope

Junya Inamoto; Shuhei Genko; Tomoyoshi Inoue; Kenzo Nishio; Osamu Matoba; Toshihiro Kubota; Yasuhiro Awatsuji

doi:10.1117/12.2615659

27 October 2021 Simultaneous three-dimensional tracking of a mother colony and a daughter colony of a moving Volvox by parallel phase-shifting digital holographic microscope

Junya Inamoto, Shuhei Genko, Tomoyoshi Inoue, Kenzo Nishio, Osamu Matoba, Toshihiro Kubota, Yasuhiro Awatsuji

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Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250H (2021) https://doi.org/10.1117/12.2615659
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

We present simultaneous three-dimensional (3D) trajectories of a mother colony and a daughter colony of a Volvox moving in water by a parallel phase-shifting digital holographic microscope. The mother colony was detected as a circle in the reconstructed phase image of the Volvox. While a daughter colony was detected as a circle in the reconstructed amplitude image of the Volvox. The lateral position of each colony was determined as the center of each circle. The depth position of each colony was determined as the position that provided the sharpest reconstructed amplitude image of the edge of each colony during the refocusing of the reconstructed image of the Volvox. We successfully trajected each colony dynamically moving in an area of 0.8 mm × 0.8 mm × 1 mm.

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Junya Inamoto, Shuhei Genko, Tomoyoshi Inoue, Kenzo Nishio, Osamu Matoba, Toshihiro Kubota, and Yasuhiro Awatsuji "Simultaneous three-dimensional tracking of a mother colony and a daughter colony of a moving Volvox by parallel phase-shifting digital holographic microscope", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250H (27 October 2021); https://doi.org/10.1117/12.2615659

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KEYWORDS

Digital holography

3D image reconstruction

Microscopes

Holograms

Holography

Phase shifts

Cameras

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