Visualization of the important components in tumor microenvironment of breast cancer using multiphoton microscopy

Jiajia He; Xingxin Huang; Deyong Kang; Liqin Zheng; Gangqin Xi; Lianhuang Li; Jianxin Chen

doi:10.1117/12.2606495

24 November 2021 Visualization of the important components in tumor microenvironment of breast cancer using multiphoton microscopy

Jiajia He, Xingxin Huang, Deyong Kang, Liqin Zheng, Gangqin Xi, Lianhuang Li, Jianxin Chen

Author Affiliations +

Proceedings Volume 12067, AOPC 2021: Biomedical Optics; 120670C (2021) https://doi.org/10.1117/12.2606495
Event: Applied Optics and Photonics China 2021, 2021, Beijing, China

Abstract

Tumor microenvironment (TME) is composed of tumor cells, stromal cells and the extracellular matrix (ECM), that it plays an important role in the occurrence and development of tumors, such as promoting local drug resistance, immune escape, and distal metastasis. Based on second harmonic generation (SHG) and two-photon excited fluorescence (TPEF), multiphoton microscopy (MPM) has the ability to label-freely visualize extracellular matrix and cells in the TME. In addition, combined SHG and TPEF imaging can be used to generate similar pathological images, providing additional information for pathologists and even surgeons. Cancer cells, adipocytes, microvessel, collagen fibers, and tumor-infiltrating lymphocytes (TILs) which were the important components in the TME were imaged using MPM in this study. The results showed that MPM can clearly present the tissue structure and cell morphology in the microenvironment. With the development and widely used of MPM, in the future, MPM imaging may be able to perform clinical imaging of the tumor microenvironment without the need for invasive operations. MPM may become a novel imaging tool for imaging various prognostic factors in breast cancer.

Citation Download Citation

Jiajia He, Xingxin Huang, Deyong Kang, Liqin Zheng, Gangqin Xi, Lianhuang Li, and Jianxin Chen "Visualization of the important components in tumor microenvironment of breast cancer using multiphoton microscopy", Proc. SPIE 12067, AOPC 2021: Biomedical Optics, 120670C (24 November 2021); https://doi.org/10.1117/12.2606495

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available