Visualizing and quantifying cisplatin-induced nephrotoxicity using nonlinear optical imaging

Aneesh Alex; Eric J. Chaney; Crysthiane Ishiy; Ciarán Fisher; Darold Spillman Jr.; Marina Marjanovic; Jan Roger; Reid Groseclose; Steve Hood; Stephen A. Boppart

doi:10.1117/12.2649232

17 March 2023 Visualizing and quantifying cisplatin-induced nephrotoxicity using nonlinear optical imaging

Aneesh Alex, Eric J. Chaney, Crysthiane Ishiy, Ciarán Fisher, Darold Spillman Jr., Marina Marjanovic, Jan Roger, Reid Groseclose, Steve Hood, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 12357, Visualizing and Quantifying Drug Distribution in Tissue VII; 1235705 (2023) https://doi.org/10.1117/12.2649232
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We investigated the potential of Simultaneous Label-free Autofluorescence Multiharmonic (SLAM) microscopy for quantitative evaluation of cisplatin-induced nephrotoxicity in rats. To determine the structural and functional changes associated with cisplatin treatment occurring over a period of time, rats were euthanized at different time points post-treatment (days 2, 6 and 29), and SLAM images were collected from 4% PFA-fixed sagittal kidney sections. Signs of renal tubular injury including hyaline cast formations were detected in SLAM images obtained from day 6 and 29 time points. This study demonstrated the capability of SLAM for visualization and evaluation of cisplatin-induced nephrotoxicity in a label-free manner.

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Aneesh Alex, Eric J. Chaney, Crysthiane Ishiy, Ciarán Fisher, Darold Spillman Jr., Marina Marjanovic, Jan Roger, Reid Groseclose, Steve Hood, and Stephen A. Boppart "Visualizing and quantifying cisplatin-induced nephrotoxicity using nonlinear optical imaging", Proc. SPIE 12357, Visualizing and Quantifying Drug Distribution in Tissue VII, 1235705 (17 March 2023); https://doi.org/10.1117/12.2649232

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