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17 March 2023 Impact of tumor microenvironment in antibody-HER2 binding using macroscopy fluorescence lifetime FRET in vivo imaging
Amit Verma, Vikas Pandey, Jason T. Smith, Xavier Intes, Margarida Barroso
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Proceedings Volume 12357, Visualizing and Quantifying Drug Distribution in Tissue VII; 123570D (2023) https://doi.org/10.1117/12.2650382
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Our goal is to accelerate pre-clinical drug discovery by developing novel imaging assays to screen and optimize the delivery of targeted anti-cancer drugs. Fluorescence lifetime imaging (FLI) Forster Resonance Energy Transfer (FRET) acts as a direct reporter of drug-target engagement in live mice carrying HER2-overexpressing tumor xenografts. We have established near-infrared (NIR) Macroscopy FLI FRET (MFLI-FRET) non-invasive imaging approach to measure drug-target engagement in deep tissues. We used trastuzumab (TZM), an anti-HER2 antibody clinical drug, as NIR-labeled FRET probes to assess quantitatively the role of tumor microenvironment on drug-target binding and penetration in tumor xenografts.
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Amit Verma, Vikas Pandey, Jason T. Smith, Xavier Intes, and Margarida Barroso "Impact of tumor microenvironment in antibody-HER2 binding using macroscopy fluorescence lifetime FRET in vivo imaging", Proc. SPIE 12357, Visualizing and Quantifying Drug Distribution in Tissue VII, 123570D (17 March 2023); https://doi.org/10.1117/12.2650382
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KEYWORDS
Tumors
Fluorescence resonance energy transfer
In vivo imaging
Luminescence
Collagen
Multiplexing
Near infrared
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