Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use

Sergey M. Zaytsev; Walter Blondel; Marine Amouroux; Valery V. Tuchin; Elina A. Genina

doi:10.1117/12.2649736

7 March 2023 Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use

Sergey M. Zaytsev, Walter Blondel, Marine Amouroux, Valery V. Tuchin, Elina A. Genina

Author Affiliations +

Proceedings Volume 12378, Dynamics and Fluctuations in Biomedical Photonics XX; 1237807 (2023) https://doi.org/10.1117/12.2649736
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The clinical use of optical methods for in vivo skin imaging is limited by skin strong scattering properties, which reduce image contrast and probing depth. The efficiency of optical methods can be improved by optical clearing (OC). However, for the use of optical clearing agents (OCAs) in a clinical setting, compliance with acceptable non-toxic concentrations is required. Optical clearing of in vivo human skin, combined with physical and chemical methods to enhance skin permeability to OCAs, was performed to determine the clearing-effectiveness of biocompatible OCAs using Line-field Confocal Optical Coherence Tomography (LC-OCT) imaging. Nine types of OCAs mixtures were used in association with dermabrasion and sonophoresis for optical clearing protocol on three volunteers hand skin. From 3D images obtained every five minutes for 40 minutes, the intensity and contrast parameters were extracted to assess their changes during the clearing process and evaluate each OCAs mixture’s clearing efficacy. LC-OCT images average intensity and contrast increased over the entire skin depth with all OCAs. The best image contrast and intensity improvement was observed using the Polyethylene Glycol, Oleic Acid and Propylene Glycol mixture. Complex OCAs featuring reduced component. concentrations that meet drug regulation-established biocompatibility requirements were developed and proved to induce. significant skin tissues clearing. By allowing deeper observations and higher contrast, such OCAs in combination with physical and chemical permeation enhancers may improve LC-OCT diagnostic efficacy.

Conference Presentation

Citation Download Citation

Sergey M. Zaytsev, Walter Blondel, Marine Amouroux, Valery V. Tuchin, and Elina A. Genina "Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use", Proc. SPIE 12378, Dynamics and Fluctuations in Biomedical Photonics XX, 1237807 (7 March 2023); https://doi.org/10.1117/12.2649736

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available