Varying laser pulse widths influence infrared neural stimulation efficacy in vitro

Jacob Hardenburger; Bryan Gamboa; Joel Bixler; Christopher Valdez; Bryan Millis; E. Duco Jansen; Anita Mahadevan-Jansen

doi:10.1117/12.3004830

13 March 2024 Varying laser pulse widths influence infrared neural stimulation efficacy in vitro

Jacob Hardenburger, Bryan Gamboa, Joel Bixler, Christopher Valdez, Bryan Millis, E. Duco Jansen, Anita Mahadevan-Jansen

Proceedings Volume 12829, Optogenetics and Optical Manipulation 2024; 1282905 (2024) https://doi.org/10.1117/12.3004830
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Infrared neural stimulation (INS) is a label-free optical neurostimulation technique that offers increased spatial specificity over traditional neurostimulation techniques. Initial studies on calcium signaling in the rat cortex demonstrated a multipart response indicating INS induces signaling in both neurons and astrocytes. We further characterized this response in cortical astrocytes in vitro, showing that different INS pulse structures induce varying levels of calcium signaling in these cells. Here we evaluate the effects of varying laser pulse parameters in cultured cortical neurons. We hypothesize that reducing the pulse duration will lead to lower stimulation thresholds. We show that INS induces a calcium response in vitro cortical neurons dependent on the total radiation exposure and time, indicating that the peak power of the infrared pulse influences INS efficacy. In conclusion, INS efficacy in vitro depends on the laser pulse width parameters.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Jacob Hardenburger, Bryan Gamboa, Joel Bixler, Christopher Valdez, Bryan Millis, E. Duco Jansen, and Anita Mahadevan-Jansen "Varying laser pulse widths influence infrared neural stimulation efficacy in vitro", Proc. SPIE 12829, Optogenetics and Optical Manipulation 2024, 1282905 (13 March 2024); https://doi.org/10.1117/12.3004830

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KEYWORDS

In vitro testing

Infrared radiation

Pulse signals

Pulsed laser operation

Neurons

Calcium

Infrared imaging

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