DNA-nanomachine-based chemiluminescent assay for pathogen detection in food borne infections

Pavel Filatov; Daria Gorbenko; Mikhail Viskov; Tigran Vartanyan

doi:10.1117/12.3022611

20 June 2024 DNA-nanomachine-based chemiluminescent assay for pathogen detection in food borne infections

Pavel Filatov, Daria Gorbenko, Mikhail Viskov, Tigran Vartanyan

Proceedings Volume 13008, Biophotonics in Point-of-Care III; 130080Q (2024) https://doi.org/10.1117/12.3022611
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

This study presents a system for the detection of S.aureus NCTC 8325 (S.aureus), using a peroxidase-like DNA nanomachine (PxDm), which has the potential to revolutionize the detection of food pathogens, as it allows for the rapid and accurate identification of unique nucleic acids. PxDm has four binding arms that can efficiently bind and unwind nucleic acid, resulting in the formation of a G-quadruplex (G-4) structure when bound to the analyte. The G-4 structure, along with hemin, forms a DNAzime with peroxidase activity, which enhances the chemiluminescence (CL) of luminol during oxidation by hydrogen peroxide molecules. The rapid and highly selective nature of this diagnostic procedure was demonstrated by evaluating the kinetics of CL within a few minutes. PxDm has numerous advantages over standard diagnostic procedures, including the ability to detect analytes at room temperature and distinguish single nucleotide substitutions. This technology could potentially turn into a laboratory device on a chip for detecting nucleic acid markers of food pathogens.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Pavel Filatov, Daria Gorbenko, Mikhail Viskov, and Tigran Vartanyan "DNA-nanomachine-based chemiluminescent assay for pathogen detection in food borne infections", Proc. SPIE 13008, Biophotonics in Point-of-Care III, 130080Q (20 June 2024); https://doi.org/10.1117/12.3022611

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