Viscoelastic properties of human colorectal cancer cells with different migration activity

Liubov E. Shimolina; Yuri M. Efremov; Nadezhda I. Ignatova; Alexander A. Gulin; Irina N. Druzhkova; Vladislav I. Shcheslavskiy; Marina K. Kuimova; Peter S. Timashev; Marina V. Shirmanova

doi:10.1117/12.3026232

18 June 2024 Viscoelastic properties of human colorectal cancer cells with different migration activity

Liubov E. Shimolina, Yuri M. Efremov, Nadezhda I. Ignatova, Alexander A. Gulin, Irina N. Druzhkova, Vladislav I. Shcheslavskiy, Marina K. Kuimova, Peter S. Timashev, Marina V. Shirmanova

Author Affiliations +

Proceedings Volume 13010, Tissue Optics and Photonics III; 1301008 (2024) https://doi.org/10.1117/12.3026232
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Biophysical properties of living cells largely determine their vital activity and functionality. In cancer, the physical state of the plasma membrane of cells is important for the invasion and metastasis. Cellular-scale viscoelasticity affects cell morphology, motility, interaction with the extracellular matrix, and resistance to mechanical stress. However, the links between membrane fluidity and cellular mechanics are poorly understood. Here, we present the in vitro study of microviscosity and viscoelastic properties of colorectal cancer cells. Measuring microviscosity of membranes at the micrometer scale was performed using fluorescence lifetime imaging microscopy FLIM with a viscosity sensitive probe. Atomic force microscopy AFM was used to evaluate the mechanical properties of cells. Additionally, the lipid profile of cells plasma membranes was analyzed using time-of-flight secondary ion mass spectrometry. A good positive correlation was found between cell stiffness (the Young’s modulus) and the plasma membrane microviscosity of cancer cells. Of the five cell lines, HT29 cells, which has an epithelial phenotype, had the most fluid membranes and the lowest stiffness values; the highest microviscosity and stiffness values were recorded for the SW480 cell line, which is characterized by a mesenchymal phenotype. The obtained results indicate that cell biomechanics is determined by the two sets of parameters that are interconnected in tumor cells and are involved in their migratory behavior.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Liubov E. Shimolina, Yuri M. Efremov, Nadezhda I. Ignatova, Alexander A. Gulin, Irina N. Druzhkova, Vladislav I. Shcheslavskiy, Marina K. Kuimova, Peter S. Timashev, and Marina V. Shirmanova "Viscoelastic properties of human colorectal cancer cells with different migration activity", Proc. SPIE 13010, Tissue Optics and Photonics III, 1301008 (18 June 2024); https://doi.org/10.1117/12.3026232

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